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Part:BBa_K957008:Design

Designed by: Steven Tiwen Chen & Dylan Patrick Webster   Group: iGEM12_Cornell   (2012-10-28)


Arsenic inducible polyHis-MtrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BamHI site found at 491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers were designed such that the wild-type Shine Dalgarno sequence was included upstream of the mtrB start codon, maintaining spacing. However, the appended BamHI cutsite downstream of the Shine Dalgarno sequence may alter translational efficiency.


Source

Arsenic-sensitive component of this composite part was originally derived from the arsenic detoxification operon of E. coli strain JM109; BioBrick part BBa_J33201 was the source of DNA.

mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.

References